Blackbox Bio

The Machine Learning Box

Automated, high-throughput behavioral analysis for in vivo research. Objective, machine-learning-driven rodent behavior screening, from a single recording.

The Platform

Two instruments. One standard.

Blackbox One instrument

Blackbox One

The Standard for In Vivo Behavior

Our original rodent imaging instrument for high-fidelity, streamlined data acquisition. Capture body video and paw print data for a single mouse or rat, or run four animals at once with the optional 4-chamber setup.

  • 30 × 30 cm open field
  • Optional 4-chamber setup
  • Up to 4 units per workstation
Blackbox Two instrument
New

Blackbox Two

Most Advanced In Vivo Platform Ever Created

Totally redesigned to enable a range of new capabilities. Everything in the Blackbox One, plus a larger recording area with improved sensitivity and an available 3D Module with four additional lateral cameras.

  • 40 × 40 cm recording area
  • 3D Module: 5 simultaneous views
  • Facial expression & 3D kinematics
  1. 1

    Roam freely, in the dark

    Rodents move naturally inside an opaque black chamber in apparent darkness, recorded from below with a near-infrared enhanced HD machine vision camera.

  2. 2

    Capture pose and paw contact

    Body pose is captured with a rapid pulse of NIR light, while plantar contact area and weight distribution of each paw are recorded separately by our proprietary sensor.

  3. 3

    Score behavior automatically

    The data streams combine into a high-speed, high-resolution video ethogram, revealing behaviors that are scored automatically and with high sensitivity.

Software

palmreader

Blackbox’s cutting-edge software for acquiring & analyzing rodent behavior data

Our palmreader software is a data management and analysis tool that provides access to machine-learning rodent-behavioral analysis algorithms to automate measurement of paw luminance ratios (representing relative weight borne on each paw) and body pose estimation, and enables users to train convolutional neural networks to automate human-scored behaviors of interest.

  • Supervised and unsupervised ML analysis of your datasets
  • Train networks to automate scoring of any behavior of interest
  • Built-in suite of statistical tools and video visualizations
  • Organize datasets by project and user; store and share securely
  • Remote access to your data from your dedicated Blackbox workstation
palmreader software running across several screens

Analysis

1 Recording4 Analysis Modules

Every session yields posture, weight bearing, gait and behavior data, with no extra animals and no extra runs.

01

Postural Analysis

  • Pose estimation
  • Body part distances
  • Joint angles
  • Toe spread
02

Dynamic Weight Bearing Analysis

  • Left vs. right
  • Forepaws vs. hindpaws
  • Paw area
03

Gait Analysis

  • Stride length & step width
  • Duty factors
  • Spatial & temporal symmetry
  • Velocity
04

Automated Behavioral Scoring

  • Grooming
  • Licking & scratching
  • Rearing
  • Locomotion

Scientific Applications

Objective endpoints, across research areas

Blackbox enables objective behavioral analysis across a wide range of preclinical research areas.

Pain & Somatosensory Research

Quantify pain states and sensory dysfunction with objective endpoints.

  • Gait
  • Weight Bearing
  • Posture
  • Behavior

Orthopedics & Musculoskeletal

Assess injury and recovery across musculoskeletal models.

  • Gait
  • Weight Bearing
  • Posture
  • Activity

Spinal Cord & Neural Injury

Measure functional impairment and recovery after neural injury.

  • Gait
  • Weight Bearing
  • Posture
  • Behavior

Itch & Sensory Behavior

Automatically detect and quantify itch-related and other sensory behaviors.

  • Behavior
  • Scratching
  • Grooming
  • Posture

Therapeutic Efficacy & Drug Discovery

Measure treatment response and optimize drug candidates.

  • Efficacy
  • Dose Responses
  • Recovery
  • Behavior

Safety, Toxicology & Tolerability

Identify and quantify adverse effects and treatment tolerability.

  • Behavior
  • Activity
  • Gait
  • Posture

Neuromuscular Disease

Evaluate motor function and disease progression in neuromuscular models.

  • Gait
  • Posture
  • Strength
  • Activity

Neurodegeneration & Motor Function

Characterize motor deficits and disease progression in neurodegenerative models.

  • Gait
  • Posture
  • Tremor
  • Activity

Cognition & Social Behavior

Investigate cognitive functions and social interactions.

  • Social Interaction
  • Exploration
  • Memory
  • Behavior

Trusted Worldwide

Labs using Blackbox

Over 100 laboratories across academia, government and pharma rely on Blackbox.

  • University of Zurich
  • Ulm University
  • University of South Carolina
  • Orion Pharma
  • Rush University
  • Cleveland Clinic
  • Myologica
  • Medical College of Wisconsin
  • Department of Veteran Affairs
  • Brixton Bio
  • Columbia University
  • Ohio State University
  • University of Copenhagen
  • University of Oulu
  • University of California Los Angeles
  • University of Michigan
  • University of Sydney
  • Xyzagen
  • University of Minnesota
  • Harvard Department of Stem Cell and Regenerative Biology
  • Michigan State University
  • Queen's University
  • Salk Institute for Biological Studies
  • Department of Defense
  • Brigham and Women's Hospital
  • Boston Children's
  • University of Texas at Dallas
  • UT Southwestern Medical School
  • University of Texas Medical Branch
  • Rutgers
  • St. Vincent's University Hospital Elm Park
  • McGill University
  • University of New England
  • Penn State
  • University of Louisville
  • University of Toronto
  • National Institutes of Health
  • University of Helsinki
  • Massachusetts General Hospital
  • Universite Clermont Auvergne
  • Boston University
  • Tafalgie
  • University of California San Francisco

Interested in learning more?

We are transforming rodent behavior screening by partnering with researchers to deliver automated, high-throughput behavioral analysis that enables deeper insights and more reproducible results. Contact us to learn how Blackbox can support your lab.

Heatmap of a mouse paw print showing weight distribution